Course at glance
Advanced Optical Fluorescence Microscopy 2 deals with quantitative fluorescence
microscopy and spectroscopy techniques that are of interest for investigators in biophysics,
biology, biomedical engineering and materials science.
Instructor
Luca Lanzanò
luca.lanzano@iit.it
Credits: 3
Synopsis
This course considers, as starting point, fundamental aspects of fluorescence spectroscopy
(absorption/ emission spectra, lifetime, energy transfer, intensity fluctuations, etc) that are
the basis of advanced fluorescence microscopy techniques. An overview of quantitative
fluorescence-based methods, including Forster Resonance Energy Transfer (FRET),
Fluorescence Lifetime Imaging (FLIM), Fluorescence Correlation Spectroscopy (FCS), will be
the core of the course. The course will focus on the principles, the experimental aspects and
examples of applications of the techniques. The methods of analysis will also be discussed.
Syllabus
The Course develops in about 9 hours in the classroom.
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Quantitative Fluorescence Spectroscopy
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Overview of advanced fluorescence spectroscopy/microscopy methods
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Critical discussion related to the biological, medical or materials science questions
Final examination: to be defined
Specific textbook for this Course:
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Molecular Fluorescence: Principles and Applications, Bernard Valeur, Wiley-VCGH Verlag
GmbH
Venue
IIT - Via Morego, 30 16163 Genova
Course date
May 2015
- Docente: LUCA LANZANO